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. 2013 Jul 12;36(2):145–150. doi: 10.1007/s10059-013-0117-7

Fig. 2.

Fig. 2.

Schematic view of the transgenic constructs. Col10a1 is located on chromosome 17 and is composed of 3 exons with the translational start codon present in exon 2 as depicted. The first construct contains 2 predicted Runx2-binding sites and includes the col10a1 promoter region from the −230 position to the +98 position, relative to the transcriptional start site (TSS) fused to the GFP open reading frame (A). The second construct contains 3 putative Runx2-binding sites and includes a ∼1.3-kb promoter fragment (B). The third construct contains 3 Runx2-binding sites and an additional ∼900-bp region upstream of the second construct, yielding an ∼2.2-kb col10a1 promoter region (C). The arrows in (C) indicate a primer set to initially isolate the promoter region by PCR. Runx2-binding sites are marked by red bars. (D–E’) Microinjection of transposase mRNA together with the construct (A) or (B) did not induce transient GFP expression at 1 dpf or 4 dpf as indicated.