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. 2013 Nov 28;36(6):507–517. doi: 10.1007/s10059-013-0149-z

Fig. 1.

Fig. 1.

Integrity of lipid rafts is required for A375 cell spreading and migration on fibronectin. (A) Time-lapse images of spreading on fibronectin of control, 5 mM MβCD-treated and 1 mM cholesterol-replenished A375 cells. Scale bar = 10 μm. (B) Kymograph analysis showed the membrane protrusion dynamics along the white lines generated from the time-lapse images shown in (A). (C) Analyses of relative membrane protrusion velocity calculated from kymographs. Values are normalized to the membrane protrusion velocity of the control cells. (D) Photomicrographs show the effect of MβCD treatment or cholesterol replenishment on the migration of A375 cells 16 h after seeding in the Trans-well upper chamber. Scale bar = 50 μm. (E) Relative number of cells transmigrated onto membrane undersurface was analyzed. Values are normalized to the relative OD value of the control cells. (F) Photomicrographs show the migration of control, MβCD-treated and cholesterol-replenished A375 cells in the agarose drop model 36 h after seeding. The dashed lines indicate the edge of the agarose drop. The arrows point the direction and distance of cell migration. Scale bar = 100 μm. (G) The distance of cell migration was measured. Each bar represents the mean ± SD from three independent experiments (Student’s t-test).