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Gain-of-function studies of trb3. Forced expression of trb3 induces ventralization of the embryos (A–H). Control embryos (A, C), injected embryos (B, D) are at 11 hpf and 36 hpf, respectively. (E–H) Molecular marker studies with eve1 (E, F) and chd (G, H); control embryos (E, G). Quantification of molecular marker study of trb3 overexpressed embryos (I).
