Fig. 2.

Dose-dependent activation of SRF-RE by LGR5, but not LGR4 or LGR6. (A) 293T cells were transfected with the SRF-RE reporter and the indicated amounts of hLGR5-Myc, and serumstarved for 24 h, followed by assay of luciferase activity. (B) hLGR4, hLGR5, and hLGR6 were overexpressed, together with the SRFRE reporter in 293T cells, as indicated. Serum-starved cells were lysed, and luciferase activity of reporters was assayed and normalized to pRL-TK Renilla luciferase activity. (C, D) 293T cells were transfected for 48 h with 2 μg plasmid encoding hLGR4, hLGR5, or hLGR6, lysed, and analyzed by western. Blots are representative of three independent experiments.