Fig. 3.
G12/13-Rho axis mediates LGR5 signaling. 293T cells were transfected with plasmid encoding LGR5 and the SRF-RE reporter and serum- starved for 24 h, followed by incubation for 4 h in the presence or absence of 1 μg/ml C3 transferase and Rho N19 mutant (A) and control (scr) siRNA or siRNA targeting Gα12 or Gα13 (B). Luciferase activity of reporters was normalized to pRL-TK Renilla luciferase activity. Values represent the mean and standard deviation from two independent experiments. (C) Efficiency of Gα12 or Gα13 knockdown by the indicated siRNAs was assessed by western analysis. Blots are representative of three independent experiments.