Fig. 4.
Downstream targets of Rho GTPase are regulated by LGR5. (A, B) HEK293 cells stably expressing hLGR5-Myc (A) and HT-29 (B) cells were transfected with control (scr) siRNA or siRNA targeting LGR5 for 48 h, followed by lysis and western analysis. Blots are representative of three independent experiments. (C-F) 293T cells were transfected with reporter plasmids c-fos-luc (C, D) or NF-κB-luc (E, F). Cells were serum-starved for 24 h, followed by incubation for 4 h in the presence or absence of 1 μg/ml C3 transferase and Rho N19 mutant (C, E) and control (scr) siRNA or siRNA targeting Gα12 or Gα13 (D, F). Luciferase activity of reporters was normalized to pRL-TK Renilla luciferase activity. Values represent the mean and standard deviation from two independent experiments.