Fig. 2.

Overexpression of SLAT in BMMs inhibits osteoclast formation. (A, B) BMMs were transduced with pMX-IRES-EGFP (control) or SLAT retrovirus and cultured for 4 days with M-CSF alone or M-CSF and various concentrations of RANKL as indicated. (A) Cultured cells were fixed and stained for TRAP. (B) Numbers of TRAP⁺ multinucleated cells were counted (#P < 0.05, *P < 0.01, ** P < 0.005 vs Control. ns, not significant). (C-F) BMMs were transduced with pMX-IRES-EGFP (control) or SLAT retrovirus and cultured with MCSF and RANKL for the indicated times. Realtime PCR analysis was performed to detect the indicated genes in control or SLAT-over-expressing samples.