Fig. 4.

SLAT is involved in RANKL-induced JNK activation. (A) BMMs were transduced with control (pMX-IRES-EGFP) or SLAT retrovirus. (B) BMMs transfected with GFP or SLAT siRNA were cultured for 2 days in the presence of M-CSF. (A, B) Cells were stimulated with RANKL (500 ng/ml) for the indicated times. Whole cell extracts were subjected to Western blot analysis with specific antibodies as indicated. (C, D) BMMs transfected with control or SLAT siRNA were cultured with M-CSF and RANKL in the absence or presence of SP600125 (5 μM). (C) Cultured cells were fixed and stained for TRAP. (D) Real-time PCR analysis was performed with the primers specific for NF ATc1 and G APDH. * P < 0.05 vs control.