Fig. 2.

Influence of S82 mutants on microtubule homeostasis. Free and polymerized tubulin fractions were prepared and probed with anti-α-tubulin antibodies. Immunoblots and relative optical density analysis in (A) indicated a decrease in polymeric tubulin, while in (B) an increase in monomeric tubulin in either H9c2 or HeLa cell lines was observed. (C, D), Western blot experiments show the influence of S82E and S82A mutants on polymeric tubulin and monomeric tubulin compared with Con group under normoxia (N) and Hypoxia (H1h) conditions in both cell lines. Histograms to the right represent the quantified relative integrated signals of H9c2 and HeLa, respectively. GAPDH in monomeric tubulin fractions was chosen as an internal control for free tubulin and VDAC in polymeric tubulin fractions was chosen for polymerized tubulin. Data are presented as mean ± SEM (n = 3). #P < 0.05 vs. N, &P < 0.05 vs. Con.