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mPT induction probed by calcein-AM/CoCl2 co-staining. Mutagenesis of S82 has an impact on hypoxia-induced permeabilization. Fluorescent micrographs of H9c2-(A) and HeLa (B) cell lines (Con, S82E and S82A groups) under normoxia and hypoxia conditions show distinct degrees of mPT induction. Graphs to the right represent the calcein fluor reduction. Values are mean ± SEM (n = 3). #P < 0.05; *P < 0.01 vs. N; &P < 0.05 vs. Con.
