Figure 7.

The influence of bafilomycin A1 on the rate of pH recovery after an episode of cytoplasmic acidification. BCEFC-loaded NPE cells were subjected to an episode of acidification by exposure for 5 minutes to Krebs solution containing 20 mM ammonium chloride; then the rate of cytoplasmic pH recovery was measured. One group of cells was superfused throughout the experiment with CO₂/HCO₃⁻-buffered Krebs solutions containing bafilomycin A1 (100 nM); a second group was superfused with bafilomycin A1 (100 nM) + acetazolamide (500 μM); and a third group was exposed to control CO₂/HCO₃⁻-buffered Krebs solutions. The results are the mean ± SEM of five to seven individual experiments. ***Significant difference from the control group, P < 0.001.