Figure 10.

Comparison of the effect of KH7 on pH recovery responses in HEK293 cells and sAC-transfected HEK293 cells (4-4 cells). BCEFC-loaded cells were subjected to an episode of acidification by exposure for 5 minutes to CO₂/HCO₃⁻-buffered Krebs solution containing 20 mM ammonium chloride; then the rate of cytoplasmic pH recovery was measured in normal CO₂/HCO₃⁻-buffered Krebs solution with or without KH7 (50 μM) added throughout the course of the experiment. The results are the mean ± SEM of five or six individual experiments. **Significant difference between control and KH7-treated 4-4 cells, P < 0.01; ###Significant difference between control HEK293 and 4-4 cells, P < 0.001.