Fig. 1.

Spatial and temporal expression patterns of patched-2 during medaka early gonad formation and in adult gonads. A patched-2 BAC reporter transgenic medaka line expressing GFP was established to follow patched-2 expression dynamics in vivo during gonad formation. Although expressed early on during somatogenesis (A), patched-2 expression was never detected during the early phase of gonadogenesis at hatching stage (B and C). In the young ovary, patched-2 expression is restricted to somatic cells of the ovarian cord (D–H) where it is co-expressed together with sox9b (I–K).