Fig. 3.

Real-time PCR expression patterns of Hedgehog and Wnt pathway components and in vivo reporter expression of R-spondin1 in medaka adult testis. Expression patterns of different components of the Hedgehog (A) and Wnt (B) pathways in organs of adult male and female medaka determined from pooled (3–4 animals) total RNA extracts. In adult testes, background levels of R-spondin1 expression are detected either by real-time PCR (B) or BAC reporter fluorescence (C and D) methods.