Table II.
gp10044–59 and APLs | Amino acid sequence | Substituted position | IC50 (nM) |
---|---|---|---|
WT | WNRQLYPEWTEAQRLD | NA | 627 |
L48F | ----F----------- | P1* | 280 |
Y49M | -----M---------- | P2 | 521 |
P50A | ------A--------- | P3 | 189 |
E51A | -------A-------- | P4* | 199 |
E51Q | -------Q-------- | P4* | 200 |
W52A | --------A------- | P5 | 372 |
T53V | ---------V------ | P6* | 489 |
E54L | ----------L----- | P7* | 65 |
E54T | ----------T----- | P7* | 104 |
A55G | -----------G---- | P8 | 553 |
Q56A | ------------A--- | P9* | 276 |
Q56I | ------------I--- | P9* | 926 |
Indicates MHC anchor residues according to crystal structure. IC50, 50% inhibitory concentration. WT, wild type gp100 peptide sequence. NA, not applicable.