Figure 2.

The protease activity of papain has adjuvant activity and leads to Th2 cell development on day 4 post immunization. (a) 4get mice were immunized with the indicated stimuli and CD4⁺DX5^- cells were examined for IL-4-eGFP expression. (b) 4get mice were immunized with papain or heat inactivated papain and Th2 differentiation was examined on indicated days post immunization. (c) CD4⁺ cells from DO11.10x4get mice were transferred into Balb/c mice, immunized as indicated and KJ1.26⁺CD4⁺ T cells were examined for IL-4-eGFP expression on day 4 post immunization, except for the OVA in Alum condition, which was examined at day 11 post immunization. Graphs and percentages are representative of multiple experiments (n>3) with 2-3 mice per group in each experiment. Error bars represent s.e.m.; p values are calculated via the Student's t test, and represent comparisons to inactive papain immunization (b) at the indicated time-point or OVA immunization (c); ***, p≤0.0001; **, p≤0.001, *, p≤0.01. If not indicated p>0.05.