Figure 5.

The protease activity of papain induces IL-4, TNF, and IL-6 production in bone marrow derived basophils. (a) IL-4 production from ≥99% purified basophils from bone marrow derived co-cultures were stimulated as indicated and intracellular cytokine staining was performed 6 hours post stimulation in vitro. (b) Histamine release by co-cultures in response to indicated stimuli 24 hours post stimulation. (c) Basophil and mast cell co-cultures were enriched for basophils by depletion of ckit⁺ cells and stimulated as indicated. Intracellular cytokine staining was performed as indicated 6 hours post stimulation in vitro. (d) IL-4 production by intracellular cytokine staining in basophil enriched co-cultures with or without serum. (e) Gene expression by Q-PCR analysis 4 hours following various stimuli. Percentages indicate that of gated population out of total basophil population. Data in (c) from triplicate samples in the experiment; error bars represent s.e.m. Data are representative of multiple (n>3) experiments.