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. 2013 Jul 11;22(23):4768–4783. doi: 10.1093/hmg/ddt330

Figure 2.

Figure 2.

Oxygen consumption and activities of mitochondrial respiratory enzymes. (A) OCR of intact cells measured polarographically. OCR is normalized to total cellular protein. Both basal and uncoupler (FCCP)-stimulated respiration rates are lower in Mclk1 knockout (KO) MEFs in comparison with controls. Results are the mean ± SEM of three paired MEF lines. (B) CS activity in whole-cell extracts normalized to total protein. Results are the mean ± SEM of five paired MEF lines. A lower activity of CS in Mclk1 KO MEFs indicates reduced mitochondrial content. (C and D) Activities of mitochondrial respiratory complexes. Mitochondria prepared from Mclk1 KO MEFs have normal activity of Complexes I–III and decreased activities of Complexes II–III and Complex II. Results are the mean ± SEM of four to five pairs of MEF lines. Paired t-tests were conducted to compare the measurements between control and Mclk1 KO MEFs. The asterisk (*) denotes a statistically significant difference at P < 0.05.