Figure 4.

Rescuing ability of various UQ analogues on galactose-induced lethality. Conditions were the same as described for Figure 3B. Cell viability is expressed as relative percentage compared with that of wild-type control fibroblasts (Mclk1^loxP/loxP-EV) treated at the same time with ethanol (vehicle). UQ₄, UQ₆ and UQ_10, but not idebenone, decylubiquinone and Vitamin K2, greatly improve the survival of Mclk1 KO fibroblasts (Mclk1^Δ/Δ-Cre) in galactose medium. Indicated doses are the highest doses that did not show significant toxicity on the control fibroblasts (idebenone, decylubiquinone and Vitamin K2) or were the most effective dose among those used in this work (UQ₄, UQ₆, UQ₉ and UQ₁₀). Data are the mean values ± SEM for at least three MEF lines. In each group (wild-type control or KO), compound treatments are compared with the ethanol-treated culture using one-way ANOVA with Dunnet's multiple comparison test. ***P < 0.001.