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Blockade of Smad3 increases myotubes size in Lmna−/− Lap2α−/−. (A) MHC immunostaining showing decreased myotubes in Lmna−/− cultures, compared to both WT and Lmna−/−Lap2α−/−. Treatment with SIS3 (bottom panels) increases myotube size. Scale, 50 μm. (B) Quantitation of nuclei per myotubes in SIS3-treated and control (CTRL) cultures (n = 6 non-overlapping fields from 2 independent experiments. *,P < 0.05; **,P < 0.01).
