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. 2013 Sep 6;5(6):391–403. doi: 10.1093/jmcb/mjt035

Figure 3.

Figure 3

Expression of E2F1 and VEGFR-3 in tumor cells is crucial to stimulate tubule formation of HUVECs. (A–D) HUVECs seeded on matrigel were cultured with conditioned media from tumor cells overexpressing E2F1 or treated with sh.E2F1 (A), sh.VEGFR-3, DN-VEGFR-3 (B), wild-type VEGFR-3 (C), or 4OHT in the presence of sh.VEGFR-3 (D). (E) Add-back experiment where VEGFR-3 and VEGF-C were restored to parental levels in E2F1 knockdown cells. Capillary tube formation was monitored by phase contrast microscopy and quantified by ImageJ (scale bar, 100 µm). Each panel shows representative images. Quantitative data are shown as bar graphs. Asterisks indicate statistical significance (P < 0.05) calculated by two-sided t-test.