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. 2013 Sep 6;5(6):391–403. doi: 10.1093/jmcb/mjt035

Figure 5.

Figure 5

E2F1 and VEGFR-3 establish a positive feedback loop in tumor cells that promotes PDGF-B expression. (A and B) Detection of VEGF-C, VEGFR-3, E2F1, and PDGF-B mRNA (A) and protein (B) levels in MCF7 and SK-Mel-28 cells cultured under normoxic or hypoxic conditions. (C) Mutual regulation of E2F1 and VEGF-C/VEGFR-3 and the effect on PDGF-B expression in MCF7 cells are shown by E2F1 knockdown using sh.E2F1 (left) and selective inhibition of VEGFR-3 signaling using 10 µM MAZ51 (right). (D and E) Impact of VEGFR-3 knockdown (D, left), VEGFR-3 overexpression (D, right), and VEGF-C(Cys156Ser) treatment at 50 ng/ml (E) on endogenous E2F1 and PDGF-B levels. (F) Analysis of PDGF-B protein level in E2F1-depleted melanoma cells exposed to Cys156Ser. Actin was used for equal loading.