Data shown are comparison of livers of mice fed a chow diet, a high fat diet, and a high fat diet with SIRT7 reintroduced specifically in the liver via AAV8-mediated gene transfer.
(A) Quantitative PCR analyses for gene expression of ER stress, inflammation, and lipogenesis.
(B) Western blotting for ER stress markers.
(C) Morphology.
(D) H&E staining, Oil Red O staining, and F4/80 staining.
(E) Quantification of liver triglyceride.
(F) A VLDL-TG secretion assay.
n=6. Error bars represent S. E. M. **:p<0.01. ***:p<0.001.