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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2013 Nov 22;98(1):411–424. doi: 10.1007/s00253-013-5394-8

Figure 3. Identification of SCFAs in the fermented media of skin microorganisms.

Figure 3

(a) Skin microorganisms (105 CFU/ml) were incubated in rich medium in the absence (M) and presence (G+M) of glycerol for six days under anaerobic conditions. Rich medium plus glycerol without skin microorganisms (G) was included as a control. Fermented media of skin microorganisms were centrifuged and passed through a 0.2 μm filter. Supernatants were then mixed with 10% D2O and analyzed by NMR spectrometers. Representative 1-D 1H- (b) and 13C- (c) NMR spectra (400 MHz) that reveal the principal SCFAs in the fermented media six days after addition of 13C3-glycerol. (d) A 2-D 1H-13C HSQC NMR spectrum (600 MHz) was displayed. In addition to glycerol (Gly), ethanol (E), alanine (A), four SCFAs [acetic acid (Ac), butyric acid (B), lactic acid (L), and succinic acid (S)] were detected in the ferments of skin microorganisms.