Table 1.

Estimate of mutation levels by subcloning

Weeks	Treatment	WT	Mutant	Total	Mutation (%)	P-value
0	None	23	75	98	76.5	N/A
4	Vehicle	53	93	146	63.4	<0.0001
	Rapamycin	99	36	135	26.7
10	Vehicle	52	45	97	46.4	<0.0001
	Rapamycin	96	11	107	10.3
16	Vehicle	66	33	99	33.3	<0.0001
	Rapamycin	126	6	132	4.5

The presence or absence of the G11778A mutation was determined in 97–146 colonies for each set of conditions, including untreated cybrids and those treated with rapamycin (20 nm) or vehicle for 4, 10 and 16 weeks. Rapamycin induced a highly significant decrease in the mutational burden at all measured time points. Data were summarized from three separate experiments and analyzed by Fisher's exact test.