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. Author manuscript; available in PMC: 2014 Jan 10.
Published in final edited form as: Mol Pharmacol. 2008 Mar 27;73(6):1709–1721. doi: 10.1124/mol.108.045591

Fig. 7.

Fig. 7

Modifications of Cys side chains introduced into 2 pore entrance positions differentially affected the “activator” effects of PD307243 and NS1643. Currents were elicited by different voltage clamp protocols to 1) illustrate their gating behavior as WT-like or mutant and 2) probe for drug effects on both outward and inward currents through the channels. For the sake of simplicity, individual voltage-clamp protocols are not shown; only the step voltages are noted (holding voltage was −80 mV in all cases). A, T613C manifested WT-like gating behavior under the control conditions (gray trace), and switched to the mutant behavior after MTSET modification (black trace). B, S631C manifested WT-like behavior (gray trace) when DTT treated, but switched to mutant behavior (black trace) after H2O2 treatment with pulsing. C and D, PD was effective in increasing currents through the T613C and S631C channels in both WT-like and mutant behavior. On the other hand, NS was effective in increasing currents through the T613C and S631C channels in the WT-like mode but incapable of increasing currents when the channels were in the mutant mode.