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. 2014 Jan 10;9(1):e84203. doi: 10.1371/journal.pone.0084203

Figure 5. The expression of Sl-ARF family genes in response to auxin and ethylene.

Figure 5

(A) Auxin induction of Sl-ARF genes on light grown seedlings. Quantitative RT-PCR of Sl-ARF transcripts in RNA samples extracted from 12-day-old tomato seedlings soaked in liquid MS medium with 10 µM IAA for 2 hours. ΔΔCT refers to the fold of difference in Sl-ARF expression to the untreated seedlings. The SAUR gene was used as control to validate the auxin treatment.(B) Ethylene regulation of Sl-ARF genes on dark grown seedlings. Quantitative RT-PCR of Sl-ARF transcripts in RNA samples extracted from5-days dark-grown tomato seedlings treated 5 hours with ethylene (50 µL/L). ΔΔCT refers to fold differences in Sl-ARF expression relative to untreated seedlings. The E4 gene was used as control for efficient ethylene treatment.