Table 4. Profile of male offspring on day 200.

	CC	CD	DD	DC
	n = 6	n = 6	n = 6	n = 6
Body weight (g) day 21	36±6	31±6	31±3	32±3
day 100	389±21	363±13	389±17	448±8*
day 200	418±7	419±15	430±12	472±18**
Heart rate (bpm)	400±15	401±12	394±10	384±12
Systolic BP (mmHg)	115±5	110±3	113±4	112±7
11β-HSD1 (pg/ml)	30.3±12.3	49.6±9.3	34.7±9.4	48.3±15.6
Adiponectin (µg/ml)	5.62±0.72	7.58±0.34*	7.30±0.45*	7.28±0.54*
Leptin (ng/ml)	6.36±0.72	6.90±0.47	7.38±0.45	7.93±0.52
Corticosterone (ng/ml)	580±26	556±24	628±18	615±23
Insulin (ng/ml)	2.21±0.10	2.46±0.20	3.02±0.20**	2.90±0.19*
Glucose (mmol/L)	9.1±0.7	9.9±0.6	12.6±1.8*	15.6±2.7**
HOMA-IR	20.6±0.1	25.0±0.1	39.1±0.4**	46.4±0.5**
HOMA-β (%)	182±26	178±20	153±22	111±26*
iCa (mmol/L)	1.39±0.02	1.33±0.03	1.37±0.03	1.38±0.04

CC in which offspring of control-diet dams were nursed by control dams; DD in which offspring of Ca-deficient-diet dams were nursed by Ca-deficient-diet dams; CD in which offspring of control-diet dams were nursed by Ca-deficient-diet dams; and DC in which offspring of Ca-deficient-diet dams were nursed by control dams. No pups remained with their original birth mother; the pups in all groups came from different litters. BP: blood pressure

Statistically significant differences among the groups were determined with two-way analysis of variance (ANOVA) with diet and sex as the main factors. If interactions were found, the data were split, and a one-way ANOVA was performed. *P<0.05, **P<0.01 vs. CC.

Values are represented as means ± SE.