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. 2014 Jan 9;8(1):e2637. doi: 10.1371/journal.pntd.0002637

Figure 3. Production of chemokines by MOPV- and LASV-infected DC cocultured with T cells and correlation with type I IFN synthesis.

Figure 3

(A) Levels of CC chemokine and CXCL11 mRNA were quantified relative to GAPDH mRNA levels, after 2 days of coculture of mock- (white bars), MOPV- (gray bars), or LASV- (black bars) infected iDC and autologous T cells. Levels of CXCL10 mRNA were evaluated in iDC/T cell coculture 2 days after each stimulation of the T cells with infected or inactivated virus-stimulated iDC. Results are expressed as the mean ± standard error (SE) of 4 independent experiments. Significant differences are indicated as follows: * (p<0.05) and ** (p<0.01). (B) The levels of CC and CXC chemokines were quantified by ELISA in the supernatants after 2 days of coculture of mock- (white bars), MOPV- (gray bars), or LASV- (black bars) infected iDC and autologous T cells. Results are expressed in pg/ml, except for CXCL10 expressed in ng/ml. Significant differences are indicated as follows: * (p<0.05). (C) Correlation between CCL4, CCL7, CXCL10, and CXCL11 mRNA levels and type I IFN synthesis by MOPV- or LASV-infected iDC cultured with naïve T cells 2 days after infection, represented by a linear regression with a correlation coefficient r and a probability of correlation p.