Figure 6. Production of CXC chemokine mRNA in PBMC and lymph nodes from LASV-infected cynomolgus monkeys.

(A) The synthesis of CXC chemokine mRNA was assessed by RT-qPCR, with normalization against β-actin mRNA in PBMC obtained from fatally infected monkeys (red squares), the low dose-infected survivor (green squares) and the high dose-infected survivors (blue circles), at various times after infection. The mRNA levels in the two mock-infected animals are represented as the mean (solid line) ± SE (hashed line) of the values obtained throughout follow-up (n = 14). (B) The production of these mRNAs was also quantified in lymph nodes obtained 9 days after infection. The levels of mRNA are represented as the mean ± SE of 4 samples for mock-infected animals (two animals, lymph nodes obtained 9 days after infection and at necropsy) and as individual values for the two fatally infected monkeys (fatal inf.), the low dose (10³ FFU)-infected survivor and the three high dose (10⁷ FFU)-infected survivors. The mean value ± SE for all infected animals is shown, together with the significant difference between mock- and LASV-infected animals. C) Correlation between CXCL9, CXCL10 and CXCL11 mRNA levels in PBMC and type I IFN levels in plasma during the course of LASV infection in cynomolgus monkeys, represented by a linear regression with a correlation coefficient r and a probability of correlation p.