Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 6;20:24–37.

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

PMC Copyright notice

Phosphatidylinositol 3-kinase (PI-3K)//Akt inhibition suppresses hepatocyte growth factor (HGF)– and keratinocyte growth factor (KGF)–mediated effect on p27^kip expression. Rabbit corneal primary epithelial (RCPE) cell growth cultures at 80%–90% confluence were starved overnight with Dulbecco’s Modified Eagle Medium with Ham’s F-12 (DMEM/F-12)/0.25% fetal calf serum (FCS) and then pretreated with PI-3K/Akt inhibitor (LY294002, 20 μM) and Erk inhibitor (PD98059, 20 μM) for 30 min before 20 ng/ml HGF) or KGF was added. Cultures were incubated for various time points as indicated, and the levels of (A) p27^kip and (C) phospho Akt (pAkt) in cellular extracts were determined with western immunoblotting. Densitometric quantification of p27^kip levels is presented in B. Data shown are mean±standard deviation (SD). p<0.05, *HGF versus LY+HGF, #KGF versus LY+KGF.