Figure 6.

Changes in the expression of apoptosis and cell cycle regulating proteins during Staurosporine (STS)-induced apoptosis in the presence of hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF). Rabbit corneal primary epithelial (RCPE) cell cultures in Dulbecco’s Modified Eagle Medium with Ham’s F-12 (DMEM/F-12)/0.25% fetal calf serum (FCS) were pretreated with 20 ng/ml HGF or (KGF) for 30 min before STS was added. Cultures were further incubated in the presence of STS (10 ng/ml) for 2 h. Then the medium was removed, and fresh medium containing HGF or KGF but no STS was added, and incubation continued for 22 h. Levels of and poly(adenosine diphosphate-ribose) polymerase (PARP), p53, and Rb proteins cellular extracts were determined with western immunoblotting by using antibodies specific for each protein (A). Experiments were performed two to three times. Quantification of different proteins is shown in bar diagrams (B–D). Data shown are mean±standard deviation (SD). *p<0.05, control versus STS, #p<0.05, STS versus STS+KGF in in (B); *p<0.05, control versus STS or STS+HGF, #p<0.05, STS versus STS+KGF in (C and D).