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. 2014 Jan 6;20:38–45.

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Copyright © 2014 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Challenge of human corneal epithelial cells with Pseudomonas aeruginosa induced differential changes in relative levels of interleukin-6, interleukin-8 and complement proteins mRNA compared to lipopolysaccharide or unchallenged control. Human corneal epithelial cells (HCEC) were challenged with Pseudomonas aeruginosa or lipopolysaccharide (LPS) for up to 3 h and subsequently cultured for up to 15 h. Data are presented as mean and standard deviation based on three independent experiments. A: Relative levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) mRNA (mRNA) in HCEC normalized to housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH, p<0.0001 (“***”) for the comparison of 18 h versus control and versus 1 h; one-way ANOVA with Bonferroni's Multiple Comparison Test). B: Relative levels of complement proteins mRNA in HCEC normalized to housekeeping genes GAPDH, beta glucuronidase and phosphoglycerate kinase 1.