Table 1. PCR primer sequences and details of TaqMan gene expression and genotyping assays.
| Gene | Primer sequence (5′-3′) | SNP Database/GenBank ID | Assay reference |
|---|---|---|---|
| MBL2 -X/Y |
rs7096206 |
C__27858274_10 |
|
| MBL2 -B (codon 54) |
rs1800450 |
C___2336609_20 |
|
| MBL2 -C (codon 57) |
rs1800451 |
C___2336608_20 |
|
| MBL2 -D (codon 52) |
rs5030737 |
C___2336610_10 |
|
| GAPDH |
F: ATGGAAATCCCATCACCATCTT |
NM_002046.4 |
|
| R: CGCCCCACTTGATTTTGG |
|||
| IL-6 |
F:CCACACAGACAGCCACTCAC |
NM_000600.3 |
|
| R: AGGTTGTTTTCTGCCAGTGC |
|||
| IL-8 |
F: CCAGGAAGAAACCACCGGA |
NM_000584.3 |
|
| R: GAAATCAGGAAGGCTGCCAAG |
|||
| MBL2 |
NM_000242.2 |
Hs00175093_m1 |
|
| GAPDH | NM_002046.4 | Hs99999905_m1 |
Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MBL, mannose-binding lectin; IL, interleukin.