Histochemical analysis of cartilage. Images are representative of alcian blue, elastin, fibrillin I, and III staining of frozen adult conchal bowl, pediatric microtia, pediatric preauricular cartilage remnants, and adult costal cartilage. All samples demonstrated glycosaminoglycans in the extracellular matrix. The TE and auricular cartilage showed similar elastin and fibrillin I distributions, with rib minimally demonstrating these proteins. Fibrillin III was positively expressed in TE samples, while not present in endogenous tissues.