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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Immunity. 2013 Nov 14;39(5):885–898. doi: 10.1016/j.immuni.2013.10.011

Figure 1. Temporal and Differential Regulation of Exudate Leukocyte miR-466l during Self-limited versus Delayed Inflammation Resolution.

Figure 1

Zym was injected (i.p.) for acute peritonitis into male FVB mice divided into three groups: high-dose (10 mg/mouse) challenged, low-dose (1 mg/mouse), and RvD1-treated.

(A) Lavages were collected at indicated intervals, and PMNs were enumerated. See Experimental Procedures and Table S1 for the calculation of resolution indices.

(B) Exudates from 1 and 10 mg of Zym-initiated murine peritonitis (0–48 hr) were collected for IL-10.

(C) Flow cytometry for apoptotic PMNs (Ly6G+AnxAV+).

(D) Flow cytometry for efferocytosis (F4/80+Ly6G+).

(E) Total RNAs were isolated at the indicated intervals, and expression of miR-466l was assessed by quantitative PCR (qPCR).

(F) miR-466l expression in each group at 24 hr.

(G and H) Quantitative expression of miR-466l in FACS-sorted PMNs (G), monocytes, and macrophages (H) from murine exudates. Results are expressed as mean ± SEM from at least four independent experiments for each point in each panel. *p < 0.05, **p < 0.01, and ***p < 0.001 versus low-dose group.

See also Figure S1 and Table S1.