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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Immunity. 2013 Nov 14;39(5):885–898. doi: 10.1016/j.immuni.2013.10.011

Figure 3. miR-466l Mediates Resolution-Phase Macrophage Polarization.

Figure 3

(A) M1 and M2 macrophages were obtained from human peripheral-blood mononuclear cells. VC and miR-466l were transfected into human macrophages for 72 hr (37°C). Phenotypes were assessed by flow cytometry after multicolor staining with fluorescent-conjugated antibodies. Relative fluorescence units (RFU) are normalized against VC and expressed as mean ± SEM from four independent experiments. *p < 0.05 and **p < 0.01, miR-466l versus VC.

(B) FVB mice were transfected with VC or miR-466l (48 hr) and then challenged with 1 mg Zym for 48 hr. Lavage peritoneal cells stained with three fluorescent-conjugated antibodies were analyzed by flow cytometry analysis. Representative flow cytometry histograms are shown. RFU are normalized against VC and expressed as mean ± SEM from four independent experiments. *p < 0.05 and **p < 0.01, versus VC.

See also Figure S3 and Table S2.