Figure 5.
Human α-lactalbumin made lethal to tumour cells (HAMLET) prophylaxis reduces intestinal tumour development. (A) ApcMin/+ mice were provided with HAMLET (10 mg/day) supplemented in the drinking water for 10 weeks and killed at 15 weeks of age. (B) Dissection photomicrographs of small intestinal segments showing prevention of tumour development (arrowheads) in HAMLET-treated mice (n=10) compared with sham-treated mice (n=10). (C) Methylene blue-stained whole mounts of intestinal segments shows reduced tumour (arrowheads) load in HAMLET-treated mice. (D) Quantification of the tumour number and size after HAMLET prophylaxis. HAMLET-treated mice (n=10) showed significant reduction (p<0.0001 for total tumour count) compared with sham-treated mice (n=10) (two-way analysis of variance test). Error bars represent ±SEM. (E) Representative immunostained sections demonstrating reduced β-catenin, cyclin D1 and COX-2 levels in HAMLET-treated tumours compared with mock-treated tumours (scale bars, 20 μm).