Table 1.

Retention times, UV, and mass spectral data of phenolic acids, flavonols, and flavan-3-ols in Hypericum perforatum dark-grown (HR1) and photoperiod-exposed (HR2) hairy root culture extracts^a.

Peak no.	Compounds	t R (min)	UV (nm)	[M–H]–  (m/z)	–MS2  [M–H]–  (m/z)	HR1 (mg·100 g−1 DW ± S.D.)	HR2 (mg·100 g−1 DW ± S.D.)
Phenolic acids
F1	Quinic acid	3.9	262, 310	191	173, 127	166.77 ± 1.20	233.14 ± 19.31
F3	3-p-Coumaroylquinic acid	19.9	314	337	191, 163	n.d.	12.18 ± 0.92
F5	3-Feruloylquinic acid	25.3	314	367	193	n.d.	5.87 ± 0.26
Flavonols
F8	Quercetin 6-C-glucoside	33.9	256, 356	421	331, 301	2.99 ± 0.79	1.74 ± 0.11
F10	Isorhamnetin O-hexoside	38.1	254, 356	477	316, 315, 271	11.80 ± 0.94	1.74 ± 0.09
F11	Kaempferol hexoside	41.2	256, 266, 350	447	285	n.d.	1.99 ± 0.26
F12	Hyperoside (quercetin 3-O-galactoside)	43.8	264, 296 sh, 354	463	301	n.d.	2.77 ± 0.18
F13	Rutin (quercetin 3-O-rutinoside)	44.9	263, 298 sh, 356	609	301	14.72 ± 2.16	5.46 ± 0.43
F14	Quercetin acetylglycoside	48.1	254, 298, 358	505	463, 445, 301	n.d.	3.94 ± 0.10
F15	Kaempferol	59.5	256, 266, 350	285	/	3.92 ± 0.38	6.26 ± 0.37
Flavan-3-ols
F2	Catechin	19.5	280	289	245, 205	27.28 ± 3.20	2.62 ± 0.08
F6	(Epi)catechin	29.9	280	289	245, 205	184.85 ± 12.92	133.36 ± 15.19
F4	Proanthocyanidin dimer	24.5	280	577	559, 451, 425, 407, 289	146.95 ± 9.13	56.61 ± 2.65
F7	Proanthocyanidin dimer	33.4	280	577	559, 451, 425, 407, 289	41.43 ± 1.03	0.76 ± 0.08
F9	Proanthocyanidin dimer	36.8	280	577	559, 451, 425, 407, 289	29.24 ± 2.47	24.93 ± 0.15

^an.d.: not detected; DW: dry weight; sh: shoulder; t _R: retention time. MS² ions in bold indicate the base peak. For information on peak numbers, see Figure 1.