Figure 3.

Administration of Tim-1 vector increased the frequencies of Tim-1⁺ cells. The Tim-1 vector was injected intraperitoneally at 10, 30, and 50 μg/mouse into Nor mice twice at 2 day intervals to observe up-regulation of Tim-1 expression. The day of the last injection, the frequencies of Tim-1⁺ cells in LN were compared to the control vector injected group by FACS analysis. The control was injected with the pCDEF3 empty vector (10 or 50 μg/mouse) (n = 3-4) (**P < 0.05). Con: control vector injection to normal (Nor) mice, Tim-1: Tim-1 vector injection to Nor mice.