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. Author manuscript; available in PMC: 2014 Jan 12.
Published in final edited form as: Am J Physiol Endocrinol Metab. 2006 Aug 22;292(1):E166–E174. doi: 10.1152/ajpendo.00284.2006

Fig. 4.

Fig. 4

Indirect coculture with primary murine macrophages blocks insulin action in adipocytes. A: insulin-stimulated glucose uptake in 3T3-L1 adipocytes cocultured with peritoneal macrophages (PM) or stromal vascular fraction (SVF) cells from epididymal adipose tissue from C57Bl/6 mice. Cells (105) of PM or SVF were added to cell culture insert, which was cultured over differentiated 3T3-L1 cells in the lower chamber. After 48 h of coculture, glucose uptake was assessed with (filled bars) and without (open bars) insulin stimulation. Data are expressed as means (SD) of triplicate determinants. Similar results were obtained from 2 independent experiments. *P < 0.05. B: cytokine analysis of media from SVF and adipocyte coculture. Media from control 3T3-L1 cultures (open bars) and indirect cocultures of SVF with 3T3-L1 adipocytes (filled bars) were collected and examined for proinflammatory cytokines TNF-α, IL-6, MIP-2, and MCP-1; n = 5 samples/condition. *P < 0.05.