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. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: J Am Chem Soc. 2013 Sep 18;135(39):14496–14499. doi: 10.1021/ja405400k

Figure 1.

Figure 1

(a) Current approach to study impacts of reactive electrophiles on protein of interest (POI) involves treatment of entire cell/proteome with a reactive entity, unavoidably leading to multiple off-target effects. Red, HNE. (b) A new approach to interrogate with biochemical specificity effects of electrophiles on individual proteins. The strategy focuses on a time-resolved targetspecific delivery of electrophiles to a single POI against the entire cell/proteome. The synthetic delivery platform ‘dormant electrophile’ shown has 3 modules: gray, high affinity/specificity ligand to POI; blue, flexible linker; pink, inert warhead – a caged HNE.