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. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: J Am Chem Soc. 2013 Sep 18;135(39):14496–14499. doi: 10.1021/ja405400k

Figure 3.

Figure 3

(a) Designed ligand is cell-permeable. COS-1 cells transiently overexpressing cytosolic HaloKeap1 were treated with either (1) 5 µM HaloTag® TMR alone, or (2) 50 µM HtPH (1, Figure 2) for 1 h prior to 5 µM HaloTag® TMR. Scale bars, 20 µM. (b) Designed ligand is non-cytotoxic. Representative data from flow cytometry dye-exclusion assays on viability of HtPHA (2, Figure 2) treated (3 h) COS-1 cells. Error bars are SD (N=3).