. Author manuscript; available in PMC: 2014 Jan 12.

Published in final edited form as: J Control Release. 2012 Oct 24;165(1):62–74. doi: 10.1016/j.jconrel.2012.10.012

Table 2.

Evaluation of stability of tetanus toxoid (TT) in PLGA microspheres prepared by traditional w/o/w emulsion-solvent evaporation method.

Concentration of TT in WP (mg/mL)	TT loading (wt%)					Encapsulation efficiency (%)^a ^*
	Theoretical	Actual^*				Antigenic TT	Total TT
	Theoretical	Soluble^a,^b	Antigenic^a,^c	Insoluble^a,^b	Total^a,^b	Antigenic TT	Total TT
6.24	0.5	0.36 ± 0.03	0.12 ± 0.01	0.11 ± 0.02	0.47 ± 0.01	24.6 ± 2.2	94.0 ± 1.3^b

WP: inner water phase;

Mean ± SEM, n = 3;

: based on TT content in polymer;

: determined by modified Bradford assay

: determined by ELISA