. Author manuscript; available in PMC: 2014 Jan 12.

Published in final edited form as: J Control Release. 2012 Oct 24;165(1):62–74. doi: 10.1016/j.jconrel.2012.10.012

Table 3.

Tetanus toxoid (TT) mass loss kinetics in the presence of antigen/unencapsulated Al(OH)₃ and antigen/3.2 wt% Al(OH)₃-3.5 wt% trehalose-5 wt% DEP-PLGA microspheres (ASM-1) mixture during temperature ramping in contrast (10-38 °C).

Incubation temperature (°C) and duration (h)		Initial TT mass^a (μg)	Formulation
Incubation temperature (°C) and duration (h)			Remaining TT mass in solution^a (μg)^*		TT loaded (μg)^*
°C	h		Unencapsulated Al(OH)₃	ASM	Unencapsulated Al(OH)₃	ASM
10	3	500	18 ± 2	374 ± 14	482 ± 2	126 ± 14
	6	500	15 ± 1	338 ± 13	485 ± 1	162 ± 13
	24	500	12 ± 1	300 ± 18	488 ± 1	200 ± 18
	48	500	ND	ND	ND	ND
25	24
25	+
38	40	500	10 ± 1	99 ± 5	490 ± 1	401 ± 5

: volume = 0.5 mL; ASM: active SM PLGA microspheres;

: Mean ± SEM, n = 3; ND: Not determined