Table 2.

Independent Binding Constants Per Molecule K_I′ from the Present Study and Literature for BPEI and PLL

polymer	binding constant (M−1)	solution	pH	method	ref
BPEI 25 kDa	4.3 × 106 5.5 × 105	50 mM Mes, 50 mM Hepes, 75 mM NaCl	7.4 5.2	TCSPC with ETI, independent method	present study
BPEI 50–60 kDa	1.2 × 105	Tris-HCl buffer, ionic strength 0.03	7	luminescence (with ETI)	28
BPEI 750 kDa	(2.3 ± 1.8) × 106	5% glucose	a	ITC (25 °C)	25
BPEI 600 Da	2 × 105	0.1 mM NaCl	6	ITC, SSIS model	38
BPEI 600 Da	1.8 × 104	0.1 mM NaCl	7	ITC, SSIS model
BPEI 600 Da	1.0 × 104	0.1 mM NaCl	8	ITC, SSIS model
BPEI 600 Da	1 × 106 1 × 105	Mes or Hepes H2O	7 7	ITC ITC
BPEI 25 kDa	(5.98 ± 1.79) × 106	20 mM phosphate buffer	7.4	ITC (25 °C)	39
PLL 200 kDa	1.5 × 107 3.0 × 106	50 mM Mes, 50 mM Hepes, 75 mM NaCl	7.4 5.2	TCSPC with ETI, independent method	present study
PLLb	9.08 × 107	c	4.8	ITC	40
PLLb	1.97 × 105	d	6.8

^aNot specified.

^bSize of PLL not reported.

^c10 mM sodium cacodylate-acetic acid, 200 mM sodium chloride, and 20 mM magnesium chloride.

^d10 mM sodium cacodylate-cacodylic acid, 200 mM sodium chloride, and 20 mM magnesium chloride.