FIGURE 2.

Transgenic rice plants enhanced resistance against three rice-infecting reoviruses. (A) Phenotype of transgenic rice plants (cv. Nipponbare) that harbor the RNAi trigger sequence targeting the Rice dwarf virus (RDV) gene for Pns12 (from Shimizu et al., 2009). (B) Phenotype of transgenic rice plants that harbor the RNAi trigger sequence targeting the Rice gall dwarf virus (RGDV) gene for Pns9 (from Shimizu et al., 2012). (C) Phenotype of transgenic rice plants that harbor the RNAi trigger sequence targeting the Rice black streaked dwarf virus (RBSDV) gene for P9-1 (from Shimizu et al., 2011a). Ten-day-old transgenic rice seedlings were exposed to approximately 10–15 viruliferous vector insects per plant for 1 day and evaluate plant response to virus infection at 4 months after virus inoculation. Rice plants in pots from left to right: mock-inoculated non-transgenic rice plants (Nt) exposed to virus-free insect vectors, showing normal growth; virus-inoculated transgenic rice plants (T), showing healthy growth and fertility after inoculation; virus-inoculated non-transgenic rice plant (Nt), showing typical symptoms caused by RDV, RGDV or RBSDV infection. Bar, 30 cm.