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. 2014 Jan 13;4:409. doi: 10.3389/fmicb.2013.00409

FIGURE 4.

FIGURE 4

Transgenic rice plants with enhanced resistance against two tenuiviruses. (A) Phenotype of transgenic rice plants (cv. Nipponbare) that harbored the RNAi trigger sequence targeting the Rice stripe virus (RSV) gene for pC3 (from Shimizu et al., 2011b). (B) Phenotype of transgenic rice plants that harbored the RNAi trigger sequence targeting the Rice grassy stunt virus (RGSV) gene for pC5 at 4 months after RGSV inoculation (from Shimizu et al., 2013). Ten-day-old transgenic rice seedlings were exposed to approximately 10–15 viruliferous vector insects per plant for 1 day and evaluate plant response to virus infection. Rice plants in pots from left to right: mock-inoculated non-transgenic rice plants (Nt) exposed to virus-free insect vectors, showing normal growth; virus-inoculated transgenic rice plants (T), showing healthy growth and fertility after inoculation; virus-inoculated non-transgenic rice plant (Nt), showing typical symptoms caused by RSV or RGSV infection. Bar, 30 cm.