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. 2014 Jan 13;4:409. doi: 10.3389/fmicb.2013.00409

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Copyright © 2014 Sasaya, Nakazono-Nagaoka, Saika, Aoki, Hiraguri, Netsu, Uehara-Ichiki, Onuki, Toki, Saito and Yatou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transgenic forage rice cultivars inoculated with Rice stripe virus (RSV) and Rice dwarf virus (RDV) (from Sasaya et al., 2013). Phenotypes of transgenic forage rice cultivars Tachiaoba (A) and Tachisugata (B) that harbored the RNAi trigger sequence targeting the RSV gene for pC3 and the RDV gene for Pns12 at 5 months after RDV and RSV inoculation. Ten-day-old transgenic forage rice seedlings were exposed to approximately 15 RSV-carrying viruliferous small brown hoppers and 10 RDV-carrying leaf hoppers per plant for each 1 day, and evaluate plant response to infection with RSV and RDV at 4 months after virus inoculation. The forage rice cultivars in pots from left to right are: mock-inoculated non-transgenic forage rice cultivars (Nt) exposed to virus-free insect vectors, showing normal growth; RDV-inoculated non-transgenic forage rice cultivars (Nt), showing typical symptoms caused by RDV infection; RSV-inoculated non-transgenic forage rice cultivars (Nt), showing typical symptoms caused by RSV infection; RDV+RSV-inoculated transgenic forage rice cultivars, showing healthy growth and fertility. Bar, 30 cm.