Table 1.
Target gene for | Location/putative functionb | GenBank accession | Resistancec |
---|---|---|---|
P1 | Core particle/RNA polymerase | D90198 | Strong |
P2 | Outer particle/vector transmission | AB263418 | Absent |
P3 | Core particle/major core capsid | X54620 | Moderate |
Pns4 | Cytoplasmic fibril/intracellular movement | X54622 | Strong |
P5 | Core particle/capping enzyme | D90033 | Absent |
Pns6 | Viroplasm/movement protein | M91653 | Immune |
P7 | Core particle/nucleic acid binding | D10218 | Absent |
P8 | Outer particle/major outer capsid | D10219 | Immune |
P9 | Outer particles/unknown | D10220 | Absent |
Pns10 | Tubule structure/silencing suppressor | D10221 | Absent |
Pns11 | Viroplasm/unknown | D10249 | Strong |
Pns12 | Viroplasm/unknown | D90200 | Immune |
To evaluate any resistance to RDV infection, more than 30 rice plants from three independent lines of transgenic plants were exposed to approximately 10 viruliferous RDV-carrying viruliferous leaf hopper per plant for 1 day.
Suzuki etal. (1990a,b, 1991, 1992a,b), Suzuki (1993), Uyeda etal. (1994), Zhong etal. (2003), Li etal. (2004), Cao etal. (2005).
Immune, no symptoms developed, and no virus was detected by ELISA in inoculated rice plants through harvest; Strong, weak symptoms developed but were delayed for 2–4 weeks, but growth was almost the same as for mock-inoculated rice plants; Moderate, typical symptoms developed but were delayed 2–4 weeks, and growth was slightly stunted after RDV infection; Absent, typical symptoms developed, as severe as those of RDV-infected non-transgenic rice plants.